Anti-BCAR1 (phospho Y751) antibody (ab45486)
Key features and details
- Rabbit polyclonal to BCAR1 (phospho Y751)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BCAR1 (phospho Y751) antibody
See all BCAR1 primary antibodies -
Description
Rabbit polyclonal to BCAR1 (phospho Y751) -
Host species
Rabbit -
Specificity
The antibody detects a 130 kDa protein corresponding to the molecular mass of phosphorylated p130 Cas on SDS-PAGE immunoblots of pervanadate treated human endothelial and A431 cells, but is not observed in control cells. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Phospho-p130 Cas (Tyr-751) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 751 in rat p130 Cas. .
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Positive control
- Pervanadate treated human endothelial and A431 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-p130 Cas (Tyr-762) peptide before affinity purification using phospho-p130 Cas (Tyr-751) peptide (without carrier). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BCAR1 (phospho Y751) antibody (ab45486)Lanes 1-2 : anti-p130 Cas
Lanes 3-4 : Anti-BCAR1 (phospho Y751) antibody (ab45486) at 1/2000 dilution
Lanes 5-6 : anti-p130 Cas (Tyr-762)
Lanes 1 & 3 & 5 : human endothelial cells serum starved overnight
Lanes 2 & 4 & 6 : human endothelial cells treated with pervanadate (1 mM) for 30 minutes
Predicted band size: 93 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
