Anti-LIM Kinase 1 antibody (ab95186)
Key features and details
- Rabbit polyclonal to LIM Kinase 1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LIM Kinase 1 antibody
See all LIM Kinase 1 primary antibodies -
Description
Rabbit polyclonal to LIM Kinase 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human LIM Kinase 1 aa 597-647 (C terminal).
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Positive control
- HeLa whole cell lysate (ab150035)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab95186 was affinity purified using an epitope specific to LIMK1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LIM Kinase 1 antibody (ab95186)All lanes : Anti-LIM Kinase 1 antibody (ab95186) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 73 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-LIM Kinase 1 antibody (ab95186)ab95186 at 3 µg/mg precipitating LIMK1 from HeLa whole cell lysate.
Lane 1; HeLa whole cell lysate. Lane 2; Control IgG. 1mg of lysate used for IP and 20% of IP loaded.
Detection utilised ab95186 at 0.4 µg/ml followed by ECL with a 5 second exposure.
