Anti-BCAR1 (phospho Y165) antibody - N-terminal (ab226914)
Key features and details
- Rabbit polyclonal to BCAR1 (phospho Y165) - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BCAR1 (phospho Y165) antibody - N-terminal
See all BCAR1 primary antibodies -
Description
Rabbit polyclonal to BCAR1 (phospho Y165) - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human BCAR1 (N terminal) (phospho Y165). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: P56945 -
Positive control
- WB: HeLa whole cell extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BCAR1 (phospho Y165) antibody - N-terminal (ab226914)All lanes : Anti-BCAR1 (phospho Y165) antibody - N-terminal (ab226914) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with CIP (calf-intestinal alkaline phosphatase), whole cell extract
Lysates/proteins at 30 µg per lane.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-BCAR1 (phospho Y165) antibody - N-terminal (ab226914)Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling BCAR1 (phospho Y165) (green) using ab226914 at 1/500 dilution in ICC/IF. Nuclear counterstained with Hoechst 33342 (blue).
