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Anti-eIF1A antibody [EPR12467] (ab172623)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12467] to eIF1A
  • Suitable for: ICC/IF, IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-eIF1A antibody [EPR12467]
    See all eIF1A primary antibodies

  • Description

    Rabbit monoclonal [EPR12467] to eIF1A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human eIF1A aa 50 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P47813

  • Positive control

    • HeLa, 293T, Jurkat, and A375 cell lysates; Colonic carcinoma tissue; Pancreas tissue; A375 cells; Immunoprecipitation pellet from HeLa whole cell lysate (ab150035)

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12467

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-eIF1A antibody [EPR12467] (ab172623)
    Western blot - Anti-eIF1A antibody [EPR12467] (ab172623)
    All lanes : Anti-eIF1A antibody [EPR12467] (ab172623) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : A375 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 16 kDa

  • Immunoprecipitation - Anti-eIF1A antibody [EPR12467] (ab172623)
    Immunoprecipitation - Anti-eIF1A antibody [EPR12467] (ab172623)

    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling eIF1A with ab172923 at 1/10 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling eIF1A with ab172623 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)

    Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue labeling eIF1A with ab172623 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12467] (ab172623)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12467] (ab172623)

    Immunofluorescent analysis of A375 cells labeling eIF1A with ab172623 at 1/50 dilution (green) and counterstained with DAPI (blue)

  • Anti-eIF1A antibody [EPR12467] (ab172623)
    Anti-eIF1A antibody [EPR12467] (ab172623)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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