Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12466(B)] to eIF1A - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free
    See all eIF1A primary antibodies

  • Description

    Rabbit monoclonal [EPR12466(B)] to eIF1A - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human endometrial carcinoma tissue; Human kidney tissue; ICC/IF: HeLa and 293T cells. Flow Cyt: HeLa Cells IP: HeLa cells

  • General notes

    Ab243919 is the carrier-free version of ab177939. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab243919 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12466(B)

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human endometrium cancer tissue sections labeling eIF1A with Purified ab177939 at 1:350 dilution (1.61 µg/ml). Heat mediated antigen retrieval was performed using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)

    Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling eIF1A with Purified ab177939 at 1:100 dilution (5.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

  • Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)
    Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)

    ab177939 (purified) at 1:30 dilution (2µg) immunoprecipitating eIF1A in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab177939 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab177939 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

  • Flow Cytometry - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)
    Flow Cytometry - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling eIF1A with Purified ab177939 at 1:60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

  • Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)
    Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)

    Western blot analysis on immunoprecipitation pellet from A375 cell lysate immunoprecipitated using ab177939 at 1/10 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

    This image was generated using the unpurified version of the product. 

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)

    Immunofluorescent analysis of HeLa cells labeling EIF1AX with ab177939 at 1/50 dilution (red). DAPI nuclear staining (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

    This image was generated using the unpurified version of the product. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EIF1AX with ab177939 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

    This image was generated using the unpurified version of the product. 

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)

    Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling EIF1AX with ab177939 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177939)

    This image was generated using the unpurified version of the product. 

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)
    Anti-eIF1A antibody [EPR12466(B)] - BSA and Azide free (ab243919)

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