Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

Order now and get it on Tuesday March 02, 2021

Anti-eIF1A antibody [EPR12466(B)] (ab177939)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12466(B)] to eIF1A
Suitable for: WB, ICC/IF, IHC-P, IP, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-eIF1A antibody [EPR12466(B)]
See all eIF1A primary antibodies
Description

Rabbit monoclonal [EPR12466(B)] to eIF1A
Host species

Rabbit
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human eIF1A aa 50 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P47813
Positive control
- WB: HeLa, Jurkat, A375 and 293T cell lysates, mouse and rat brain tissue lysates; IHC-P: Human endometrial carcinoma tissue; Human kidney tissue; ICC/IF: HeLa and 293T cells. Flow Cyt: HeLa cells IP: HeLa cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12466(B)
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] (ab177939)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human endometrium cancer tissue sections labeling eIF1A with Purified ab177939 at 1:350 dilution (1.61 µg/ml). Heat mediated antigen retrieval was performed using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] (ab177939)

ab177939 (purified) at 1:30 dilution (2µg) immunoprecipitating eIF1A in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab177939 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab177939 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12466(B)] (ab177939)

Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling eIF1A with Purified ab177939 at 1:100 dilution (5.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-eIF1A antibody [EPR12466(B)] (ab177939)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling eIF1A with Purified ab177939 at 1:60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-eIF1A antibody [EPR12466(B)] (ab177939)

All lanes : Anti-eIF1A antibody [EPR12466(B)] (ab177939) at 1/10000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates
Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 4 : Mouse brain lysates
Lane 5 : Rat brain lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 16 kDa
Observed band size: 16 kDa
Western blot - Anti-eIF1A antibody [EPR12466(B)] (ab177939)

All lanes : Anti-eIF1A antibody [EPR12466(B)] (ab177939) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A375 cell lysate
Lane 4 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 16 kDa

This image was generated using the unpurified version of the product.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] (ab177939)

Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling EIF1AX with ab177939 at 1/50 dilution.

This image was generated using the unpurified version of the product.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12466(B)] (ab177939)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EIF1AX with ab177939 at 1/50 dilution.

This image was generated using the unpurified version of the product.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12466(B)] (ab177939)

Immunofluorescent analysis of HeLa cells labeling EIF1AX with ab177939 at 1/50 dilution (red). DAPI nuclear staining (blue).

This image was generated using the unpurified version of the product.
Immunoprecipitation - Anti-eIF1A antibody [EPR12466(B)] (ab177939)

Western blot analysis on immunoprecipitation pellet from A375 cell lysate immunoprecipitated using ab177939 at 1/10 dilution.

This image was generated using the unpurified version of the product.
Anti-eIF1A antibody [EPR12466(B)] (ab177939)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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