Human GC Globulin ELISA Kit (Vitamin D Binding Protein) (ab108853)
Key features and details
- Sensitivity: 0.075 µg/ml
- Range: 0.098 µg/ml - 100 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Human GC Globulin ELISA Kit (Vitamin D Binding Protein)
See all Vitamin D Binding protein kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.8% Inter-assay Sample n Mean SD CV% Overall 9.8% -
Sample type
Serum, Plasma -
Assay type
Competitive -
Sensitivity
> 0.075 µg/ml -
Range
0.098 µg/ml - 100 µg/ml -
Recovery
97 %
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Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human GC Globulin (Vitamin D Binding Protein) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of GC Globulin in plasma, serum, saliva, milk and cell-culture supernatants.
A GC Globulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated GC Globulin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of GC Globulin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml 3X Biotinylated Human GC Globulin (Lyophilized) 1 vial Chromogen Substrate 1 x 8ml GC Globulin Microplate (12 x 8 well strips) 1 unit GC Globulin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- DBP
- DBP/GC
- GC
see all -
Database links
- Entrez Gene: 2638 Human
- Omim: 139200 Human
- SwissProt: P02774 Human
- Unigene: 418497 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab108853.
