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Human Vitamin D Binding Protein ELISA Kit (ab223586)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 2.8 pg/ml
  • Range: 19.5 pg/ml - 1250 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Vitamin D Binding Protein ELISA Kit
    See all Vitamin D Binding protein kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 6 3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 4.3%

  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    2.8 pg/ml

  • Range

    19.5 pg/ml - 1250 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 99 86% - 107%
    Milk 102 93% - 108%
    Urine 102 99% - 108%
    Serum 95 91% - 99%
    Cell culture media 95 95% - 95%
    Hep Plasma 92 88% - 96%
    EDTA Plasma 101 94% - 105%
    Cit plasma 98 94% - 102%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human
    Does not react with: Cow

  • Product overview

    Human Vitamin D Binding Protein ELISA Kit (ab223586) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Vitamin D Binding Protein protein in cit plasma, edta plasma, hep plasma, milk, saliva, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Vitamin D Binding Protein with 2.8 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Vitamin D Binding Protein, also known as gc-globulin, is a 458 - amino acid, glycosylated protein encoded by the gene GC, which is in the albumin gene family. The primary function of this protein is to bind Vitamin D and its metabolites and to transport them to tissue. Vitamin D Binding protein is highly expressed and secreted into serum primarily from hepatic cells. There are three common alleles of the protein (Gc1f, Gc1s, and Gc2), and several minor polymorphisms. Different glycosylation sites are found on the isoforms of Vitamin D binding protein, a selectively deglycosylated form produced by B cell ß-galactosidase and T cell sialidase is known as Macrophage Activating Factor. MAF has been found to block angiogenic effects of several proteins on vascular endothelial cells.    

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Human Vitamin D Binding Protein ELISA Kit (ab223586)
    Other - Human Vitamin D Binding Protein ELISA Kit (ab223586)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Human Vitamin D Binding Protein Competitor standard curve comparison
    Human Vitamin D Binding Protein Competitor standard curve comparison

    Standard curve comparison between Human Vitamin D Binding Protein SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • Example of human Vitamin D Binding Protein standard curve in Sample Diluent NS.
    Example of human Vitamin D Binding Protein standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native Vitamin D Binding Protein in human serum and plasma samples.
    Interpolated concentrations of native Vitamin D Binding Protein in human serum and plasma samples.

    The concentrations of Vitamin D Binding Protein were measured in duplicates, interpolated from the Vitamin D Binding Protein standard curves and corrected for sample dilution. All undiluted samples start at 1:500,000 in Sample Diluent NS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vitamin D Binding Protein concentration was determined to be 427 µg /mL in neat serum, 380 µg /mL in neat plasma (citrate), 552 µg /mL in neat plasma (EDTA), and 271 µg/mL in neat plasma Heparin.

  • Interpolated concentrations of native Vitamin D Binding Protein in human milk, saliva, and urine samples.
    Interpolated concentrations of native Vitamin D Binding Protein in human milk, saliva, and urine samples.

    The concentrations of Vitamin D Binding Protein were measured in duplicates, interpolated from the Vitamin D Binding Protein standard curves and corrected for sample dilution. All undiluted samples start at 1:500,000 in Sample Diluent NS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vitamin D Binding Protein concentration was determined to be 5,280 ng /mL in neat milk, 288 ng/mL in neat saliva, and 56 ng /mL in neat urine.

  • Serum from ten individual healthy human female donors was measured in duplicate.
    Serum from ten individual healthy human female donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vitamin D Binding Protein concentration was determined to be 632 µg/mL with a range of 460 – 866 µg/mL.

  • Sandwich ELISA - Human Vitamin D Binding Protein ELISA Kit (ab223586)
    Sandwich ELISA - Human Vitamin D Binding Protein ELISA Kit (ab223586)
    To learn more about the advantages of recombinant antibodies see here.

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