Human Vitamin D Binding Protein ELISA Kit (ab223586)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.8 pg/ml
- Range: 19.5 pg/ml - 1250 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Vitamin D Binding Protein ELISA Kit
See all Vitamin D Binding protein kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 6 3% Inter-assay Sample n Mean SD CV% Overall 3 4.3% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.8 pg/ml -
Range
19.5 pg/ml - 1250 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 99 86% - 107% Milk 102 93% - 108% Urine 102 99% - 108% Serum 95 91% - 99% Cell culture media 95 95% - 95% Hep Plasma 92 88% - 96% EDTA Plasma 101 94% - 105% Cit plasma 98 94% - 102% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human Vitamin D Binding Protein ELISA Kit (ab223586) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Vitamin D Binding Protein protein in cit plasma, edta plasma, hep plasma, milk, saliva, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Vitamin D Binding Protein with 2.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Vitamin D Binding Protein, also known as gc-globulin, is a 458 - amino acid, glycosylated protein encoded by the gene GC, which is in the albumin gene family. The primary function of this protein is to bind Vitamin D and its metabolites and to transport them to tissue. Vitamin D Binding protein is highly expressed and secreted into serum primarily from hepatic cells. There are three common alleles of the protein (Gc1f, Gc1s, and Gc2), and several minor polymorphisms. Different glycosylation sites are found on the isoforms of Vitamin D binding protein, a selectively deglycosylated form produced by B cell ß-galactosidase and T cell sialidase is known as Macrophage Activating Factor. MAF has been found to block angiogenic effects of several proteins on vascular endothelial cells.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml 10X Human Vitamin D Binding Protein Capture Antibody 1 x 600µl 10X Human Vitamin D Binding Protein Detector Antibody 1 x 600µl Human Vitamin D Binding Protein Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- DBP
- DBP/GC
- GC
see all -
Database links
- Entrez Gene: 2638 Human
- Omim: 139200 Human
- SwissProt: P02774 Human
- Unigene: 418497 Human
Images
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Other - Human Vitamin D Binding Protein ELISA Kit (ab223586)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Vitamin D Binding Protein Competitor standard curve comparisonStandard curve comparison between Human Vitamin D Binding Protein SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human Vitamin D Binding Protein standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Vitamin D Binding Protein in human serum and plasma samples.The concentrations of Vitamin D Binding Protein were measured in duplicates, interpolated from the Vitamin D Binding Protein standard curves and corrected for sample dilution. All undiluted samples start at 1:500,000 in Sample Diluent NS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vitamin D Binding Protein concentration was determined to be 427 µg /mL in neat serum, 380 µg /mL in neat plasma (citrate), 552 µg /mL in neat plasma (EDTA), and 271 µg/mL in neat plasma Heparin.
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Interpolated concentrations of native Vitamin D Binding Protein in human milk, saliva, and urine samples.The concentrations of Vitamin D Binding Protein were measured in duplicates, interpolated from the Vitamin D Binding Protein standard curves and corrected for sample dilution. All undiluted samples start at 1:500,000 in Sample Diluent NS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vitamin D Binding Protein concentration was determined to be 5,280 ng /mL in neat milk, 288 ng/mL in neat saliva, and 56 ng /mL in neat urine.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Vitamin D Binding Protein concentration was determined to be 632 µg/mL with a range of 460 – 866 µg/mL.
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Sandwich ELISA - Human Vitamin D Binding Protein ELISA Kit (ab223586)To learn more about the advantages of recombinant antibodies see here.
