Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RRM1 antibody [SP167] (ab135383)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP167] to RRM1
  • Suitable for: Flow Cyt, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-RRM1 antibody [SP167]
    See all RRM1 primary antibodies

  • Description

    Rabbit monoclonal [SP167] to RRM1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human RRM1 aa 300-400 (internal sequence). The exact sequence is proprietary.
    Database link: P23921

  • Positive control

    • Human lung adenocarcinoma tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [SP167] (ab135383)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM1 antibody [SP167] (ab135383)

    Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labelling RRM1 with ab135383 antibody at a dilution of 1/100.

  • Flow Cytometry - Anti-RRM1 antibody [SP167] (ab135383)
    Flow Cytometry - Anti-RRM1 antibody [SP167] (ab135383)

    Flow cytometric analysis of rabbit anti-RRM1 (SP167) antibody ab135383 (1/400) in HeLa cells (green) compared to negative control of rabbit IgG (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-RRM1 antibody [SP167] (ab135383)
    Immunocytochemistry/ Immunofluorescence - Anti-RRM1 antibody [SP167] (ab135383)

    ICC/IF image of ab135383 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab135383 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-RRM1 antibody [SP167] (ab135383)
    Anti-RRM1 antibody [SP167] (ab135383)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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