Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20247] to Natriuretic peptides A
  • Suitable for: WB, IHC-P, IHC-Fr, IP
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Natriuretic peptides A antibody [EPR20247]
    See all Natriuretic peptides A primary antibodies

  • Description

    Rabbit monoclonal [EPR20247] to Natriuretic peptides A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Rat
    IHC-P
    Rat
    Human
    IP
    Rat
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal heart and rat heart tissue lysates. IHC-P: Human heart, rat auricle and rat ventricle tissues. IHC-Fr: Rat heart tissue. IP: Rat heart lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)
    Western blot - Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)
    All lanes : Anti-Natriuretic peptides A antibody [EPR20247] (ab209232) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 16 kDa
    Observed band size: 16 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 2 seconds; Lane 2: 15 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)

    Immunohistochemical analysis of paraffin-embedded human heart tissue labeling Natriuretic peptides A with ab209232 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Granularly cytoplasmic and perinuclear staining on human heart (PMID: 2942710, PMID: 1824903).

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)
    Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat heart tissue labeling Natriuretic peptides A with ab209232 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Cytoplasmic staining on the auricula of rat heart (PMID: 2942710, PMID: 1824903).

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)

    Immunohistochemical analysis of paraffin-embedded rat auricle tissue labeling Natriuretic peptides A with ab209232 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Granularly cytoplasmic and perinuclear staining on rat auricle (PMID: 2942710, PMID: 1824903).

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)

    Immunohistochemical analysis of paraffin-embedded rat ventricle tissue labeling Natriuretic peptides A with ab209232 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Weak staining on rat ventricle (PMID: 2942710, PMID: 1824903).

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)
    Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)

    Natriuretic peptides A was immunoprecipitated from 0.35 mg of rat heart lysate with ab209232 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab209232 at 1/500 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Rat heart lysate 10 µg (Input).

    Lane 2: ab209232 IP in rat heart lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209232 in rat heart lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)
    Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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