Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20247] to Natriuretic peptides A
Suitable for: WB, IHC-P, IHC-Fr, IP
Reacts with: Rat, Human

Product name

Anti-Natriuretic peptides A antibody [EPR20247]
See all Natriuretic peptides A primary antibodies
Description

Rabbit monoclonal [EPR20247] to Natriuretic peptides A
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Rat
IHC-P	Rat Human
IP	Rat
WB	Rat Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal heart and rat heart tissue lysates. IHC-P: Human heart, rat auricle and rat ventricle tissues. IHC-Fr: Rat heart tissue. IP: Rat heart lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20247
Isotype

IgG
Research areas

Western blot - Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)

All lanes : Anti-Natriuretic peptides A antibody [EPR20247] (ab209232) at 1/1000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Rat heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 16 kDa
Observed band size: 16 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 2 seconds; Lane 2: 15 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)

Immunohistochemical analysis of paraffin-embedded human heart tissue labeling Natriuretic peptides A with ab209232 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Granularly cytoplasmic and perinuclear staining on human heart (PMID: 2942710, PMID: 1824903).

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat heart tissue labeling Natriuretic peptides A with ab209232 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Cytoplasmic staining on the auricula of rat heart (PMID: 2942710, PMID: 1824903).

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)

Immunohistochemical analysis of paraffin-embedded rat auricle tissue labeling Natriuretic peptides A with ab209232 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Granularly cytoplasmic and perinuclear staining on rat auricle (PMID: 2942710, PMID: 1824903).

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)

Immunohistochemical analysis of paraffin-embedded rat ventricle tissue labeling Natriuretic peptides A with ab209232 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Weak staining on rat ventricle (PMID: 2942710, PMID: 1824903).

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)

Natriuretic peptides A was immunoprecipitated from 0.35 mg of rat heart lysate with ab209232 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab209232 at 1/500 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: Rat heart lysate 10 µg (Input).

Lane 2: ab209232 IP in rat heart lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209232 in rat heart lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.
Anti-Natriuretic peptides A antibody [EPR20247] (ab209232)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com