Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22089-283] to Natriuretic peptides A
Suitable for: IHC-Fr, IHC-P, IP, WB
Reacts with: Mouse, Rat

Product name

Anti-Natriuretic peptides A antibody [EPR22089-283]
See all Natriuretic peptides A primary antibodies
Description

Rabbit monoclonal [EPR22089-283] to Natriuretic peptides A
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat
IP	Mouse
WB	Mouse Rat

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse and rat heart tissue lysate. IHC-P: Mouse heart and heart atrium tissues; Rat heart atrium tissue. IHC-Fr: Mouse and rat heart tissues. IP: Mouse heart tissue lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22089-283
Isotype

IgG
Research areas

Western blot - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)

All lanes : Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844) at 1/1000 dilution

Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 16 kDa
Observed band size: 17 kDa
why is the actual band size different from the predicted?

Exposure time : Lane 1: 3 minutes; Lane 2: 8 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular mass observed is consistent with what has been described in the literature (PMID: 16291870).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)

Immunohistochemical analysis of paraffin-embedded mouse heart tissue labeling Natriuretic peptides A with ab225844 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Strong cytoplasmic staining mainly in the atria and at lower levels in ventricle of mouse heart (PMID: 2942710; PMID:25532015; PMID: 1824903). Counterstained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse heart tissue labeling Natriuretic peptides A with ab225844 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining in cardiac muscle of mouse atrium is observed.

The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat heart tissue labeling Natriuretic peptides A with ab225844 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining in cardiac muscle of rat atrium is observed.

The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)

Immunohistochemical analysis of paraffin-embedded mouse heart atrium tissue labeling Natriuretic peptides A with ab225844 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in cardiac muscle of mouse atrium (PMID: 2942710; PMID:25532015; PMID: 1824903). Counterstained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)

Natriuretic peptides A was immunoprecipitated from 0.35 mg Mouse heart tissue lysate with ab225844 at 1/1000 dilution. Western blot was perfromed from the immunoprecipitate using ab225844 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.

Lane 1: Mouse heart lysate 10 μg (Input).
Lane 2: ab225844 IP in Mouse heart tissue lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab225844 in Mouse heart lysate (-).

Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 10 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)

Immunohistochemical analysis of paraffin-embedded rat heart atrium tissue labeling Natriuretic peptides A with ab225844 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in cardiac muscle of rat atrium (PMID: 2942710; PMID:25532015; PMID: 1824903). Counterstained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Natriuretic peptides A antibody [EPR22089-283] (ab225844)

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