Anti-Spa-1 antibody (ab85928)
Key features and details
- Rabbit polyclonal to Spa-1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Spa-1 antibody
See all Spa-1 primary antibodies -
Description
Rabbit polyclonal to Spa-1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Spa-1 aa 992-1042.
Database link: NP_006738.3 -
Positive control
- HeLa cells, 293T and mouse NIH3T3 whole cell lysates
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General notes
Previously labelled as SIPA1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab85928 was affinity purified using an epitope specific to Spa-1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Spa-1 antibody (ab85928)
Detection of Spa-1 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab85928 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 30 seconds. -
Western blot - Anti-Spa-1 antibody (ab85928)All lanes : Anti-Spa-1 antibody (ab85928) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : mouse NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 112 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
