Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Spa-1 antibody [EPR14134] (ab189929)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14134] to Spa-1
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Spa-1 antibody [EPR14134]
    See all Spa-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14134] to Spa-1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal thymus, HeLa, Raji, HEK-293T and Raw 264.7 lysates. IHC-P: Human kidney tissue. ICC/IF: A431 cells. Flow Cyt: 293T cells.

  • General notes

     

     

    Previously labelled as SIPA1. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Spa-1 antibody [EPR14134] (ab189929)
    Western blot - Anti-Spa-1 antibody [EPR14134] (ab189929)
    All lanes : Anti-Spa-1 antibody [EPR14134] (ab189929) at 1/5000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : Spa-1 knockout HeLa cell lysate
    Lane 3 : Raji cell lysate
    Lane 4 : HEK-293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 112 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab189929 observed at 130 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab189929 Anti-Spa-1 antibody [EPR14134] was shown to specifically react with Spa-1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265434 (knockout cell lysate ab258189) was used. Wild-type and Spa-1 knockout samples were subjected to SDS-PAGE. ab189929 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spa-1 antibody [EPR14134] (ab189929)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spa-1 antibody [EPR14134] (ab189929)

    Immunohistochemical analysis of formalin fixed, paraffin embedded Human kidney tissue labeling Spa-1 with ab189929 at 1/50 dilution, and Prediluted HRP Polymer for Rabbit IgG as a secondary antibody. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Spa-1 antibody [EPR14134] (ab189929)
    Immunocytochemistry/ Immunofluorescence - Anti-Spa-1 antibody [EPR14134] (ab189929)

    Immunocytochemical analysis of A431 cells fixed in 4% paraformaldehyde labeling Spa-1 with ab189929 at 1/250 dilution, and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 dilution as the secondary antibody. Counterstained with DAPI.

  • Western blot - Anti-Spa-1 antibody [EPR14134] (ab189929)
    Western blot - Anti-Spa-1 antibody [EPR14134] (ab189929)
    All lanes : Anti-Spa-1 antibody [EPR14134] (ab189929) at 1/5000 dilution

    Lane 1 : Human fetal thymus
    Lane 2 : Raji cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 112 kDa
    Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-Spa-1 antibody [EPR14134] (ab189929)
    Western blot - Anti-Spa-1 antibody [EPR14134] (ab189929)
    Anti-Spa-1 antibody [EPR14134] (ab189929) at 1/1000 dilution + Raw264.7 at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 112 kDa
    Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands.

  • Flow Cytometry - Anti-Spa-1 antibody [EPR14134] (ab189929)
    Flow Cytometry - Anti-Spa-1 antibody [EPR14134] (ab189929)

    Flow cytometric analysis of 293T cells fixed in 2% paraformaldehyde labeling Spa-1 with ab189929 at 1/400 dilution, and Goat anti rabbit IgG (FITC) at 1/150 dilution as the secondary antibody.

  • Anti-Spa-1 antibody [EPR14134] (ab189929)
    Anti-Spa-1 antibody [EPR14134] (ab189929)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Spa-1 antibody [EPR14134] (ab189929)

© 2021 Astana Biomed Group LLP. All rights reserved.