Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MCPIP1 antibody [15D11] - BSA and Azide free (ab255884)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [15D11] to MCPIP1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-MCPIP1 antibody [15D11] - BSA and Azide free
    See all MCPIP1 primary antibodies

  • Description

    Rat monoclonal [15D11] to MCPIP1 - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    ELISA
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW264.7 (treated 4hrs with 100ng/ml LPS) whole cell lysate; F9 whole cell lysate.

  • General notes

    ab255884 is the carrier-free version of ab252879. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255884 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-MCPIP1 antibody [15D11] - BSA and Azide free (ab255884)
    Western blot - Anti-MCPIP1 antibody [15D11] - BSA and Azide free (ab255884)
    All lanes : Anti-MCPIP1 antibody [15D11] (ab252879) at 1/1000 dilution

    Lane 1 : Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 2 : RAW264.7 treated with 100ng/ml LPS (Lipopolysaccharide) for 4 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution (Goat Anti-Rat IgG (H+L), HRP)

    Predicted band size: 65 kDa
    Observed band size: 65 kDa



    This data was developed using ab252879, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 29263935).

    Exposure time 3 minutes.

  • Western blot - Anti-MCPIP1 antibody [15D11] - BSA and Azide free (ab255884)
    Western blot - Anti-MCPIP1 antibody [15D11] - BSA and Azide free (ab255884)
    Anti-MCPIP1 antibody [15D11] (ab252879) at 1/1000 dilution + F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution (Goat Anti-Rat IgG (H+L), HRP)

    Predicted band size: 65 kDa
    Observed band size: 65 kDa



    This data was developed using ab252879, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time 20 seconds.

  • Anti-MCPIP1 antibody [15D11] - BSA and Azide free (ab255884)
    Anti-MCPIP1 antibody [15D11] - BSA and Azide free (ab255884)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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