Anti-TTC19 antibody [EPR13184] - BSA and Azide free (ab250323)
![Anti-TTC19 antibody [EPR13184] - BSA and Azide free (ab250323)](https://www.abcam.com/ps/products/250/ab250323/Images/ab250323-408413-anti-ttc19-antibody-epr13184-bsa-and-azide-free-western-blot.jpg "Anti-TTC19 antibody [EPR13184] - BSA and Azide free (ab250323)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13184] to TTC19 - BSA and Azide free
- Suitable for: ICC, IP, WB
- Reacts with: Human
Overview
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Product name
Anti-TTC19 antibody [EPR13184] - BSA and Azide free
See all TTC19 primary antibodies -
Description
Rabbit monoclonal [EPR13184] to TTC19 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250323 is the carrier-free version of ab181069. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250323 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13184 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TTC19 antibody [EPR13184] - BSA and Azide free (ab250323)All lanes : Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069) at 1/2000 dilution
Lane 1 : Human fetal muscle tissue lysate
Lane 2 : Human ovarian cancer tissue lysate
Lane 3 : Human fetal heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?This data was developed using ab181069, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-TTC19 antibody [EPR13184] - BSA and Azide free (ab250323)](https://www.abcam.com/ps/products/250/ab250323/Images/ab250323-405786-anti-ttc19-antibody-epr13184-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-TTC19 antibody [EPR13184] - BSA and Azide free (ab250323)Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069) at 1/10000 dilution + HT-1080 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 42 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?This data was developed using ab181069, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry - Anti-TTC19 antibody [EPR13184] - BSA and Azide free (ab250323)](https://www.abcam.com/ps/products/250/ab250323/Images/ab250323-2-anti-ttc19-antibody-epr13184-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-TTC19 antibody [EPR13184] - BSA and Azide free (ab250323)This data was developed using ab181069, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HT-1080 cells, staining TTC19 (green) with ab181069. Goat anti rabbit IgG (Alexa Fluor®488) was used as a secondary antibody at 1/200 dilution. Counterstained with DAPI (blue) -
![Immunoprecipitation - Anti-TTC19 antibody [EPR13184] - BSA and Azide free (ab250323)](https://www.abcam.com/ps/products/250/ab250323/Images/ab250323-399613-anti-ttc19-antibody-epr13184-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TTC19 antibody [EPR13184] - BSA and Azide free (ab250323)This data was developed using ab181069, the same antibody clone in a different buffer formulation.
Western blot analysis on immunoprecipitation pellet from HT-1080, labeling TTC19 immunoprecipitated using ab181069 at 1/70 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Anti-TTC19 antibody [EPR13184] - BSA and Azide free (ab250323)](https://www.abcam.com/ps/products/250/ab250323/Images/ab250323-5-benefits-of-recombinant-antibodies.png)
Anti-TTC19 antibody [EPR13184] - BSA and Azide free (ab250323)