Anti-TTC4 antibody (ab241507)
Key features and details
- Rabbit polyclonal to TTC4
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TTC4 antibody
See all TTC4 primary antibodies -
Description
Rabbit polyclonal to TTC4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human TTC4 aa 1-50. The exact sequence is proprietary.
Database link: O95801 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241507 was affinity purified using an epitope specific to TTC4 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TTC4 antibody (ab241507)All lanes : Anti-TTC4 antibody (ab241507) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-TTC4 antibody (ab241507)TTC4 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241507 used for IP at 20 µl per reaction. Western blot was perfomed on the immunoprecipitate using ab241507 at 1/100 dilution.
Lane 1: ab241507 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 30 seconds.
