Human Galectin-1 ELISA Kit (ab260053)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.36 ng/ml
- Range: 0.8 ng/ml - 50 ng/ml
- Sample type: Cell culture media, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Rat, Cow, Human
Overview
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Product name
Human Galectin-1 ELISA Kit
See all Galectin 1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.2% Inter-assay Sample n Mean SD CV% Serum 4 5.6% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.36 ng/ml -
Range
0.8 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 97 88% - 102% Cell culture media 107 98% - 114% Hep Plasma 110 102% - 119% EDTA Plasma 103 94% - 108% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Product overview
Human Galectin-1 ELISA Kit (ab260053) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Galectin-1 protein in cell culture media, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Galectin-1 with 0.36 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Galectin-1, also known as 14 kDa lectin, galaptin, and lectin galactoside-binding soluble 1, is a β-galactoside binding lectin encoded by the gene LGALS1. Galectin-1 is a 134 amino acid non-glycosylated secreted protein that forms a non-covalently linked homodimer. Galectin-1 is expressed in a wide range of tissues and has various intra- and extra-cellular functions, where generally it is a modulator of immune responses. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Galectin-1 Capture Antibody 1 x 600µl 10X Human Galectin-1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Human Galectin-1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. -
Tissue specificity
Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. -
Sequence similarities
Contains 1 galectin domain. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- 14 kDa laminin-binding protein
- 14 kDa lectin
- Beta galactoside binding lectin
see all -
Database links
- Entrez Gene: 326598 Cow
- Entrez Gene: 3956 Human
- Entrez Gene: 16852 Mouse
- Entrez Gene: 56646 Rat
- Omim: 150570 Human
- SwissProt: P11116 Cow
- SwissProt: P09382 Human
- SwissProt: P16045 Mouse
see all
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Galectin-1 standard curve comparisonStandard Curve comparison between human RANTES SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human Galectin-1 standard curve in Sample Diluent NS.The Galectin-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Galectin-1 in human serum and plasma samples.The concentrations of Galectin-1 were measured in duplicates, interpolated from the Galectin-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-1 concentration was determined to be 58 ng/mL in serum, 52.5 ng/mL in plasma (EDTA) and 72.8 ng/mL in plasma (heparin).
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Serum from eight individual healthy human donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-1 concentration was determined to be 99.2 ng/mL with a range of 4.3 –165.5 ng/mL.
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Sandwich ELISA - Human Galectin-1 ELISA Kit (ab260053)To learn more about the advantages of recombinant antibodies see here.
