Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TTC4 antibody [EPR13381] (ab181194)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13381] to TTC4
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-TTC4 antibody [EPR13381]
    See all TTC4 primary antibodies

  • Description

    Rabbit monoclonal [EPR13381] to TTC4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293T, HepG2, MCF7 and U87-UG cell lysates; Human muscle and colon tissues; MCF7 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13381

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TTC4 antibody [EPR13381] (ab181194)
    Western blot - Anti-TTC4 antibody [EPR13381] (ab181194)
    All lanes : Anti-TTC4 antibody [EPR13381] (ab181194) at 1/50000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : U87-MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa

  • Western blot - Anti-TTC4 antibody [EPR13381] (ab181194)
    Western blot - Anti-TTC4 antibody [EPR13381] (ab181194)
    Anti-TTC4 antibody [EPR13381] (ab181194) at 1/10000 dilution + MCF7 at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC4 antibody [EPR13381] (ab181194)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC4 antibody [EPR13381] (ab181194)

    Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling TTC4 with ab181194 at 1/100 dilution. Secondary antibody: anti rabbit IgG (HRP), prediluted. Counter stain: Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC4 antibody [EPR13381] (ab181194)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC4 antibody [EPR13381] (ab181194)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TTC4 with ab181194 at 1/100 dilution. Secondary antibody: anti rabbit IgG (HRP), prediluted. Counter stain: Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TTC4 antibody [EPR13381] (ab181194)
    Immunocytochemistry/ Immunofluorescence - Anti-TTC4 antibody [EPR13381] (ab181194)

    Immunofluorescent analysis of 4% paraformaldehyde fixed MCF7 cells labeling TTC4 with ab181194 at 1/200 dilution. Secondary antibody: Goat anti rabbit IgG (Alexa Fluor®488). Conterstained with Dapi (blue).

  • Immunoprecipitation - Anti-TTC4 antibody [EPR13381] (ab181194)
    Immunoprecipitation - Anti-TTC4 antibody [EPR13381] (ab181194)

    Western blot analysis of TTC4 in immunoprecipitation pellets from 293T cell lysate.

    Lane 1: ab181194 used at 1/50 dilution. 

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    Lane 2: Negative control

  • Anti-TTC4 antibody [EPR13381] (ab181194)
    Anti-TTC4 antibody [EPR13381] (ab181194)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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