Antibodies, Proteins, Kits and Reagents for Life Science

257 980 ₸ Product size

100 µl 257 980 ₸

Order now and get it on Tuesday March 02, 2021

Anti-TTC4 antibody [EPR13381] (ab181194)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13381] to TTC4
Suitable for: WB, IHC-P, ICC/IF, IP
Reacts with: Human

Product name

Anti-TTC4 antibody [EPR13381]
See all TTC4 primary antibodies
Description

Rabbit monoclonal [EPR13381] to TTC4
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- 293T, HepG2, MCF7 and U87-UG cell lysates; Human muscle and colon tissues; MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13381
Isotype

IgG
Research areas

Western blot - Anti-TTC4 antibody [EPR13381] (ab181194)

All lanes : Anti-TTC4 antibody [EPR13381] (ab181194) at 1/50000 dilution

Lane 1 : 293T cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : U87-MG cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa
Western blot - Anti-TTC4 antibody [EPR13381] (ab181194)

Anti-TTC4 antibody [EPR13381] (ab181194) at 1/10000 dilution + MCF7 at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC4 antibody [EPR13381] (ab181194)

Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling TTC4 with ab181194 at 1/100 dilution. Secondary antibody: anti rabbit IgG (HRP), prediluted. Counter stain: Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC4 antibody [EPR13381] (ab181194)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TTC4 with ab181194 at 1/100 dilution. Secondary antibody: anti rabbit IgG (HRP), prediluted. Counter stain: Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TTC4 antibody [EPR13381] (ab181194)

Immunofluorescent analysis of 4% paraformaldehyde fixed MCF7 cells labeling TTC4 with ab181194 at 1/200 dilution. Secondary antibody: Goat anti rabbit IgG (Alexa Fluor®488). Conterstained with Dapi (blue).
Immunoprecipitation - Anti-TTC4 antibody [EPR13381] (ab181194)

Western blot analysis of TTC4 in immunoprecipitation pellets from 293T cell lysate.
Lane 1: ab181194 used at 1/50 dilution.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Lane 2: Negative control
Anti-TTC4 antibody [EPR13381] (ab181194)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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