Anti-TTC4 antibody [EPR13381-76] (ab181195)
![Anti-TTC4 antibody [EPR13381-76] (ab181195)](https://www.abcam.com/ps/products/181/ab181195/Images/ab181195-215264-ab181195.jpg "Anti-TTC4 antibody [EPR13381-76] (ab181195)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13381-76] to TTC4
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-TTC4 antibody [EPR13381-76]
See all TTC4 primary antibodies -
Description
Rabbit monoclonal [EPR13381-76] to TTC4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T, HepG2, MCF7 and U87-MG cell lysates; Human testis and Human transitional cell carcinoma of bladder tissue; MCF7 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13381-76 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TTC4 antibody [EPR13381-76] (ab181195)All lanes : Anti-TTC4 antibody [EPR13381-76] (ab181195) at 1/20000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : U87-MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa
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![Western blot - Anti-TTC4 antibody [EPR13381-76] (ab181195)](https://www.abcam.com/ps/products/181/ab181195/Images/ab181195-215266-ab1811952.jpg)
Western blot - Anti-TTC4 antibody [EPR13381-76] (ab181195)Anti-TTC4 antibody [EPR13381-76] (ab181195) at 1/5000 dilution + MCF7 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC4 antibody [EPR13381-76] (ab181195)](https://www.abcam.com/ps/products/181/ab181195/Images/ab181195-215268-ab1811953.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC4 antibody [EPR13381-76] (ab181195)Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TTC4 with ab181195 at 1/100 dilution. Secondary antibody: anti rabbit IgG (HRP), prediluted. Counter stain: Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-TTC4 antibody [EPR13381-76] (ab181195)](https://www.abcam.com/ps/products/181/ab181195/Images/ab181195-215279-ab1811955.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TTC4 antibody [EPR13381-76] (ab181195)Immunofluorescent analysis of 4% paraformaldehyde fixed MCF7 cells labeling TTC4 with ab181195 at 1/250 dilution. Secondary antibody: Goat anti rabbit IgG (Alexa Fluor® 488) at a 1/200 dilution. Conterstained with Dapi (blue).
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![Anti-TTC4 antibody [EPR13381-76] (ab181195)](https://www.abcam.com/ps/products/181/ab181195/Images/ab181195-6-benefits-of-recombinant-antibodies.png)
Anti-TTC4 antibody [EPR13381-76] (ab181195)
