Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD96 antibody [BLR065G] - BSA free (ab264416)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR065G] to CD96 - BSA free
  • Suitable for: IP, WB, IHC-P, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-CD96 antibody [BLR065G] - BSA free
    See all CD96 primary antibodies

  • Description

    Rabbit monoclonal [BLR065G] to CD96 - BSA free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human CD96 aa 29-502 (extracellular). The exact sequence is proprietary. NP_005807.1.
    Database link: P40200-2

  • Positive control

    • IHC-P: Human ovarian carcinoma and tonsil tissues. ICC: MOLT-4 cells. WB: MOLT-4, A2058, CCRF-CEM, KG-1 and HH whole cell lysates. IP: MOLT-4 whole cell lysate.

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc

     

Properties

Images

  • Immunocytochemistry - Anti-CD96 antibody [BLR065G] - BSA free (ab264416)
    Immunocytochemistry - Anti-CD96 antibody [BLR065G] - BSA free (ab264416)

    Formalin-fixed, paraffin-embedded MOLT-4 (human lymphoblastic leukemia cell line) cells stained for CD96 using ab264416 at 1/200 dilution in imunocytochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD96 antibody [BLR065G] - BSA free (ab264416)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD96 antibody [BLR065G] - BSA free (ab264416)

    Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for CD96 using ab264416 at 1/200 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

  • Immunoprecipitation - Anti-CD96 antibody [BLR065G] - BSA free (ab264416)
    Immunoprecipitation - Anti-CD96 antibody [BLR065G] - BSA free (ab264416)

    CD96 was immunoprecipitated from MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264416 at 6 µl per reaction. Western bot was performed on the immunoprecipitates using ab264416 at 1/1000 dilution.

    Lane 1: ab264416 IP in MOLT-4 whole cell lysate.
    Lane 2: Control IgG IP in MOLT-4 whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 3 seconds.

    Lysates prepared using NETN lysis buffer.

  • Western blot - Anti-CD96 antibody [BLR065G] - BSA free (ab264416)
    Western blot - Anti-CD96 antibody [BLR065G] - BSA free (ab264416)
    All lanes : Anti-CD96 antibody [BLR065G] - BSA free (ab264416) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 4 : A2058 whole cell lysate
    Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 6 : CCRF-CEM (human T cell lymphoblast cell line) whole cell lysate
    Lane 7 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 8 : KG-1 whole cell lysate
    Lane 9 : HH whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 64 kDa


    Exposure time: 30 seconds


    Lysates prepared using NETN lysis buffer.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD96 antibody [BLR065G] - BSA free (ab264416)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD96 antibody [BLR065G] - BSA free (ab264416)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD96 using ab264416 at 1/200 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

  • Anti-CD96 antibody [BLR065G] - BSA free (ab264416)
    Anti-CD96 antibody [BLR065G] - BSA free (ab264416)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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