Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CD96 antibody [BLR065G] - BSA free (ab264416)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR065G] to CD96 - BSA free
Suitable for: IP, WB, IHC-P, ICC
Reacts with: Human

Product name

Anti-CD96 antibody [BLR065G] - BSA free
See all CD96 primary antibodies
Description

Rabbit monoclonal [BLR065G] to CD96 - BSA free
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment within Human CD96 aa 29-502 (extracellular). The exact sequence is proprietary. NP_005807.1.
Database link: P40200-2
Positive control
- IHC-P: Human ovarian carcinoma and tonsil tissues. ICC: MOLT-4 cells. WB: MOLT-4, A2058, CCRF-CEM, KG-1 and HH whole cell lysates. IP: MOLT-4 whole cell lysate.
General notes

This product is sold under License from Bethyl Laboratories, Inc

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline
Concentration information loading...
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR065G
Isotype

IgG
Research areas

Immunocytochemistry - Anti-CD96 antibody [BLR065G] - BSA free (ab264416)

Formalin-fixed, paraffin-embedded MOLT-4 (human lymphoblastic leukemia cell line) cells stained for CD96 using ab264416 at 1/200 dilution in imunocytochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD96 antibody [BLR065G] - BSA free (ab264416)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for CD96 using ab264416 at 1/200 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunoprecipitation - Anti-CD96 antibody [BLR065G] - BSA free (ab264416)

CD96 was immunoprecipitated from MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264416 at 6 µl per reaction. Western bot was performed on the immunoprecipitates using ab264416 at 1/1000 dilution.

Lane 1: ab264416 IP in MOLT-4 whole cell lysate.
Lane 2: Control IgG IP in MOLT-4 whole cell lysate.

Detection: Chemiluminescence with an exposure time of 3 seconds.

Lysates prepared using NETN lysis buffer.
Western blot - Anti-CD96 antibody [BLR065G] - BSA free (ab264416)

All lanes : Anti-CD96 antibody [BLR065G] - BSA free (ab264416) at 1/1000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : A2058 whole cell lysate
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 6 : CCRF-CEM (human T cell lymphoblast cell line) whole cell lysate
Lane 7 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 8 : KG-1 whole cell lysate
Lane 9 : HH whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Developed using the ECL technique.

Predicted band size: 64 kDa

Exposure time: 30 seconds

Lysates prepared using NETN lysis buffer.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD96 antibody [BLR065G] - BSA free (ab264416)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD96 using ab264416 at 1/200 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Anti-CD96 antibody [BLR065G] - BSA free (ab264416)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com