Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD96 antibody [EPR22120-174] - BSA and Azide free (ab255696)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22120-174] to CD96 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD96 antibody [EPR22120-174] - BSA and Azide free
    See all CD96 primary antibodies

  • Description

    Rabbit monoclonal [EPR22120-174] to CD96 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P,IP or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: NK-92 cells. Flow: Human peripheral blood mononuclear cells; NK-92 cells.

  • General notes

    Ab255696 is the carrier-free version of ab224687. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255696 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-CD96 antibody [EPR22120-174] - BSA and Azide free (ab255696)
    Flow Cytometry - Anti-CD96 antibody [EPR22120-174] - BSA and Azide free (ab255696)

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD96 with ab224687 at 1/60 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab224687 (Right). Then stained with anti-CD3 conjugated to Alexa Fluor® 647. 

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224687).

  • Flow Cytometry - Anti-CD96 antibody [EPR22120-174] - BSA and Azide free (ab255696)
    Flow Cytometry - Anti-CD96 antibody [EPR22120-174] - BSA and Azide free (ab255696)

    Flow cytometric analysis of NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) cells labelling CD96 with ab224687 at 1/60 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224687).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD96 antibody [EPR22120-174] - BSA and Azide free (ab255696)
    Immunocytochemistry/ Immunofluorescence - Anti-CD96 antibody [EPR22120-174] - BSA and Azide free (ab255696)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NK-92 (human peripheral blood malignant non-Hodgkin's lymphoma cell line) cells labelling CD96 with ab224687 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in NK-92 cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)  was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224687).

  • Anti-CD96 antibody [EPR22120-174] - BSA and Azide free (ab255696)
    Anti-CD96 antibody [EPR22120-174] - BSA and Azide free (ab255696)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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