Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Thursday March 04, 2021

Anti-ICAM2 antibody [EPR19114-113] (ab197570)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19114-113] to ICAM2
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-ICAM2 antibody [EPR19114-113]
See all ICAM2 primary antibodies
Description

Rabbit monoclonal [EPR19114-113] to ICAM2
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Jurkat cell lysate; human fetal kidney and fetal spleen lysate. IHC-P: Human lung and spleen tissue.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19114-113
Isotype

IgG
Research areas

Western blot - Anti-ICAM2 antibody [EPR19114-113] (ab197570)

Anti-ICAM2 antibody [EPR19114-113] (ab197570) at 1/5000 dilution + Jurkat (human T cell leukemia T lymphocyte) lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 31 kDa
Observed band size: 60 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 1676048).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR19114-113] (ab197570)

Immunohistochemical analysis of paraffin-embedded human lung tissue labeling ICAM2 with ab197570 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on vascular endothelium of human lung (PMID: 1676048) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-ICAM2 antibody [EPR19114-113] (ab197570)

All lanes : Anti-ICAM2 antibody [EPR19114-113] (ab197570) at 1/100 dilution

Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

Developed using the ECL technique.

Predicted band size: 31 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?

Exposure time: 20 seconds

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR19114-113] (ab197570)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling ICAM2 with ab197570 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on vascular endothelium of human spleen (PMID: 1676048) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-ICAM2 antibody [EPR19114-113] (ab197570)

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