Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 03, 2021

Anti-ICAM2 antibody [EPR18231-183] (ab189463)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18231-183] to ICAM2
Suitable for: WB, IHC-P, IP
Reacts with: Mouse

Product name

Anti-ICAM2 antibody [EPR18231-183]
See all ICAM2 primary antibodies
Description

Rabbit monoclonal [EPR18231-183] to ICAM2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse heart, kidney and bEnd.3 cell lysate. IHC: Mouse cerebral cortex and spleen tissue. IP: bEnd.3 cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18231-183
Isotype

IgG
Research areas

Western blot - Anti-ICAM2 antibody [EPR18231-183] (ab189463)

All lanes : Anti-ICAM2 antibody [EPR18231-183] (ab189463) at 1/1000 dilution

Lane 1 : bEnd.3 (mouse brain endothelioma cell line) cell lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 31 kDa
Observed band size: 60 kDa
why is the actual band size different from the predicted?

The molecular weight observed is consistent with what has been described in the literature (PMID: 1676048).

Blocking/Dilution: 5% NFDM/TBST

Exposure: Lane1: 5 seconds; lanes 2 and 3: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR18231-183] (ab189463)

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling ICAM2 with ab189463 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Positive staining on vascular endothelium of mouse cerebral cortex (PMID: 12516546). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR18231-183] (ab189463)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling ICAM2 with ab189463 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Positive staining on vascular endothelium of mouse spleen (PMID: 12516546). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunoprecipitation - Anti-ICAM2 antibody [EPR18231-183] (ab189463)

ICAM2 was immunoprecipitated from 0.35 mg of bEnd.3 (mouse brain endothelioma cell line) whole cell lysate with ab189463 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab189463 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: bEnd.3 whole cell lysate 10 µg (Input).

Lane 2: ab189463 IP in bEnd.3 whole cell lysate (+).

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189463 in bEnd.3 whole cell lysate (-).

Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds
Anti-ICAM2 antibody [EPR18231-183] (ab189463)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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