Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1620Y] to Cytokeratin 7
Suitable for: WB, IP
Knockout validated
Reacts with: Human

Product name

Anti-Cytokeratin 7 antibody [EP1620Y]
See all Cytokeratin 7 primary antibodies
Description

Rabbit monoclonal [EP1620Y] to Cytokeratin 7
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human Cytokeratin 7 aa 450-550 (C terminal).
Positive control
- WB: HeLa, T-47D, SK-BR-3, A549 and placenta cell lysates
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1620Y
Isotype

IgG
Research areas

Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460)

All lanes : Goat Anti-Rat IgG H&L (FITC) (ab6840) at 1/10000 dilution

Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : KRT7 knockout A549 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MCF7 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 51 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab68460 observed at 50 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab68460 was shown to specifically react with KRT7 (Cytokeratin 7) in wild-type A549 cells as signal was lost in KRT7 knockout cells. Wild-type and KRT7 knockout samples were subjected to SDS-PAGE. Ab68460 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunoprecipitation - Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460)

ab68460 (purified) at 1:20 dilution (0.6µg) immunoprecipitating Cytokeratin 7 in T-47D whole cell lysate.
Lane 1 (input): T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate 10μg
Lane 2 (+): ab68460 & T-47D whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68460 in T-47D whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460)

All lanes : Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460) at 1/20000 dilution (purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg/ml
Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates at 20 µg/ml
Lane 3 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg/ml
Lane 4 : Human placenta lysates at 20 mg/ml

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 51 kDa

Blocking and diluting buffer : 5% NFDM/TBST

~45 kDa bands are supported by the literature and competitor’s products, which may be an isoform.[PMID:16265353]
Western blot - Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460)

Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460) at 1/20000 dilution (unpurified) + HeLa cell lysate at 10 µg

Secondary
HRP conjugated Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 51 kDa
Observed band size: 51 kDa
Anti-Cytokeratin 7 antibody [EP1620Y] (ab68460)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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