Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (ab251597)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CAL67] to CD8 alpha - BSA and Azide free
  • Suitable for: IHC-P, WB, IP, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-CD8 alpha antibody [CAL67] - BSA and Azide free
    See all CD8 alpha primary antibodies

  • Description

    Rabbit monoclonal [CAL67] to CD8 alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil and colon tissue. ICC/IF: Human PBMCs. IP: IP in human thymus lysate.

  • General notes

    Ab251597 is the carrier-free version of ab237710. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251597 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (ab251597)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (ab251597)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD8 alpha with ab237710 at 1/1500 dilution (0.352 μg/ml), followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the T lymphocytes in the human colon is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237710).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (ab251597)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (ab251597)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD8 alpha with ab237710 at 1/1500 dilution (0.352 μg/ml), followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on human tonsil tissue is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237710).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (ab251597)
    Immunocytochemistry/ Immunofluorescence - Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (ab251597)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMC (human primary peripheral blood mononuclear cell) cells labeling CD8 alpha with ab237710 at 1/100 dilution (5.3µg), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in subsets of human PBMC. The nuclear counter stain is DAPI (blue). Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The negative control is the secondary antibody only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237710).

  • Immunoprecipitation - Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (ab251597)
    Immunoprecipitation - Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (ab251597)

    CD8 alpha was immunoprecipitated from 0.35 mg human thymus lysate with ab237710 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab237710 at 1/1000 (0.53µg/ml) dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
    Lane 1: Human thymus lysate 10 µg (Input).
    Lane 2: ab237710 IP in human thymus lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237710 in human thymus lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237710).

  • Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (ab251597)
    Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (ab251597)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD8 alpha antibody [CAL67] - BSA and Azide free (ab251597)

© 2021 Astana Biomed Group LLP. All rights reserved.