Anti-KIF5B antibody (ab5629)
Key features and details
- Rabbit polyclonal to KIF5B
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KIF5B antibody
See all KIF5B primary antibodies -
Description
Rabbit polyclonal to KIF5B -
Host species
Rabbit -
Specificity
This antibody is specific for KIF5B and does not detect other kinesin isotypes. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-KIF5B antibody (ab5629)
ab5629 labelling KIF5B (green) in HeLa cells. Cells fixed in 3.2% paraformaldehyde and permeabilized with 0.2% Triton X-100 for 30 minutes at 37ºC were blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:250) overnight at 4°C. A fluorescently-conjugated Goat anti-rabbit IgG was used as the secondary antibody (1:2000). Images were taken on wide-field fluorescent microscope at 63X magnification.
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Western blot - Anti-KIF5B antibody (ab5629)Shows ab5629 used for a Western blot of Mouse neuronal cell extract.
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Immunocytochemistry/ Immunofluorescence - Anti-KIF5B antibody (ab5629)ab5629 at 1/200 dilution staining the mouse oligodendrocyte cell line, Olineu, by immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy3 conjugated secondary antibody.
This image is courtesy of an Abreview submitted by Katarina Trajko on 22 February 2006.
