Anti-KIF5B antibody (ab187693)
Images
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All lanes : Anti-KIF5B antibody (ab187693) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 110 kDa
Exposure time: 30 seconds
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Detection of Kinesin Heavy Chain in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab187693 at 6 µg/mg lysate. An alternative anti-Kinesin Heavy Chain antibody was used at 1 µg/ml for subsequent western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 1 second.