Anti-FLASH antibody (ab8420)
Key features and details
- Rabbit polyclonal to FLASH
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FLASH antibody
See all FLASH primary antibodies -
Description
Rabbit polyclonal to FLASH -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide:
SERFQQLMKLFEKSKC
, corresponding to amino acids 1966/1981 of Human FLASH. (Peptide available as ab8455.) -
Positive control
- HeLa cell lysate An approximate 220 kDa band can be detected.
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General notes
A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Affinity purified -
Primary antibody notes
A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2). -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Images
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Western blot - Anti-FLASH antibody (ab8420)Anti-FLASH antibody (ab8420) at 0.5 µg/ml + HeLa whole cell lysate
Observed band size: 220 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-FLASH antibody (ab8420)ab8420 at 10µg/ml staining FLASH in Hela cells by ICC/IF
