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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Crosslinking

Anti-Filamin A (phospho S1458) antibody [EP2309Y] (ab68424)

Price and availability

227 827 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Filamin A (phospho S1458) antibody [EP2309Y] (ab68424)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit monoclonal [EP2309Y] to Filamin A (phospho S1458)
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Filamin A (phospho S1458) antibody [EP2309Y]
    See all Filamin A primary antibodies
  • Description

    Rabbit monoclonal [EP2309Y] to Filamin A (phospho S1458)
  • Host species

    Rabbit
  • Specificity

    Detects Filamin A only when phosphorylated at serine 1458.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding serine 1458 of Human Filamin A

  • Positive control

    • HeLa cells. Stomach. Uterus.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP2309Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Crosslinking

Images

  • Western blot - Anti-Filamin A (phospho S1458) antibody [EP2309Y] (ab68424)
    Western blot - Anti-Filamin A (phospho S1458) antibody [EP2309Y] (ab68424)
    All lanes : Anti-Filamin A (phospho S1458) antibody [EP2309Y] (ab68424) at 1/5000 dilution

    Lane 1 : HeLa cell lysate - untreated
    Lane 2 : HeLa cell lysate - treated with AP

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labelled Goat anti-Rabbit antibody at 1/2000 dilution

    Predicted band size: 280 kDa
    Observed band size: 260 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A (phospho S1458) antibody [EP2309Y] (ab68424)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A (phospho S1458) antibody [EP2309Y] (ab68424)
    Immunohistochemical analysis of paraffin-embedded human stomach (left) and human uterus tissue (right) using 1/50 ab68424. Heat-mediated antigen retrieval was performed prior to commencing with IHC protocol and an HRP-conjugated polymerized secondary antibody was used.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Filamin A (phospho S1458) antibody [EP2309Y] (ab68424)

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    Anti-Filamin A antibody [EP2405Y] (ab76289)

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    Anti-Filamin A antibody (ab111620)

    Applications: ICC/IF, IHC-P, WB

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    Anti-Filamin A (phospho S1083) antibody [EPR2308] (ab109269)

    Applications: WB

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    Anti-Filamin A antibody [EP2405Y] - BSA and Azide free (ab239881)

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    Anti-Filamin A antibody (ab189183)

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    Anti-Filamin A antibody (ab190300)

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    Anti-Filamin A antibody [NO.A0F7] (ab238514)

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