Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Filamin A antibody [NO.A0F7] (ab238514)

Key features and details

Rabbit monoclonal [NO.A0F7] to Filamin A
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Human
Isotype: IgG

Product name

Anti-Filamin A antibody [NO.A0F7]
See all Filamin A primary antibodies
Description

Rabbit monoclonal [NO.A0F7] to Filamin A
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human Filamin A (C terminal).
Database link: P21333

Run BLAST with Run BLAST with
Positive control
- WB: MCF7, Jurkat, HeLa and NIH/3T3 cell extracts. IHC-P: Human and mouse uterus tissues. ICC/IF: HUVEC, HeLa and Ags cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
Purity

Affinity purified
Clonality

Monoclonal
Clone number

NO.A0F7
Isotype

IgG
Research areas

Western blot - Anti-Filamin A antibody [NO.A0F7] (ab238514)

All lanes : Anti-Filamin A antibody [NO.A0F7] (ab238514) at 1/500 dilution

Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell extract
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) cell extract

Lysates/proteins at 25 µg per lane.

Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 281 kDa

Blocking buffer: 3% non-fat dry milk in TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A antibody [NO.A0F7] (ab238514)

Paraffin-embedded mouse uterus tissue stained for Filamin A using ab238514 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A antibody [NO.A0F7] (ab238514)

Paraffin-embedded human uterus tissue stained for Filamin A using ab238514 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A antibody [NO.A0F7] (ab238514)

HUVEC (human umbilical vein endothelial cell line) cells stained for Filamin A (green) using ab238514 at 1/50 dilution in ICC/IF.
Immunocytochemistry/ Immunofluorescence - Anti-Filamin A antibody [NO.A0F7] (ab238514)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Filamin A (green) using ab238514 at 1/50 dilution in ICC/IF.
Immunocytochemistry/ Immunofluorescence - Anti-Filamin A antibody [NO.A0F7] (ab238514)

Ags cells stained for Filamin A (green) using ab238514 at 1/50 dilution in ICC/IF.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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