Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Filamin A antibody [NO.A0F7] (ab238514)

Key features and details

  • Rabbit monoclonal [NO.A0F7] to Filamin A
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Filamin A antibody [NO.A0F7]
    See all Filamin A primary antibodies

  • Description

    Rabbit monoclonal [NO.A0F7] to Filamin A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human Filamin A (C terminal).
    Database link: P21333

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: MCF7, Jurkat, HeLa and NIH/3T3 cell extracts. IHC-P: Human and mouse uterus tissues. ICC/IF: HUVEC, HeLa and Ags cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    NO.A0F7

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Filamin A antibody [NO.A0F7] (ab238514)
    Western blot - Anti-Filamin A antibody [NO.A0F7] (ab238514)
    All lanes : Anti-Filamin A antibody [NO.A0F7] (ab238514) at 1/500 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell extract
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) cell extract

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size: 281 kDa



    Blocking buffer: 3% non-fat dry milk in TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A antibody [NO.A0F7] (ab238514)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A antibody [NO.A0F7] (ab238514)

    Paraffin-embedded mouse uterus tissue stained for Filamin A using ab238514 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A antibody [NO.A0F7] (ab238514)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A antibody [NO.A0F7] (ab238514)

    Paraffin-embedded human uterus tissue stained for Filamin A using ab238514 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A antibody [NO.A0F7] (ab238514)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A antibody [NO.A0F7] (ab238514)

    HUVEC (human umbilical vein endothelial cell line) cells stained for Filamin A (green) using ab238514 at 1/50 dilution in ICC/IF.

  • Immunocytochemistry/ Immunofluorescence - Anti-Filamin A antibody [NO.A0F7] (ab238514)
    Immunocytochemistry/ Immunofluorescence - Anti-Filamin A antibody [NO.A0F7] (ab238514)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Filamin A (green) using ab238514 at 1/50 dilution in ICC/IF.

  • Immunocytochemistry/ Immunofluorescence - Anti-Filamin A antibody [NO.A0F7] (ab238514)
    Immunocytochemistry/ Immunofluorescence - Anti-Filamin A antibody [NO.A0F7] (ab238514)

    Ags cells stained for Filamin A (green) using ab238514 at 1/50 dilution in ICC/IF.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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