Anti-alpha Actinin 4 antibody (ab231247)
Key features and details
- Rabbit polyclonal to alpha Actinin 4
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
-
Product name
Anti-alpha Actinin 4 antibody
See all alpha Actinin 4 primary antibodies -
Description
Rabbit polyclonal to alpha Actinin 4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat, Cow, Orangutan
-
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human alpha Actinin 4 aa 1-300. (Expressed in E.coli).
Database link: O43707 -
Positive control
- IHC-P: Human kidney, stomach, colorectal cancer, bile duct cancer and breast cancer tissues. WB: Recombinant human alpha Actinin 4 protein; MCF7 and HeLa cell lysates; Pig liver lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab231247 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-alpha Actinin 4 antibody (ab231247)All lanes : Anti-alpha Actinin 4 antibody (ab231247) at 3 µg/ml
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
Lane 2 : Pig liver lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Secondary
All lanes : HRP-linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 105 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin 4 antibody (ab231247)Paraffin-embedded human colorectal cancer tissue stained for alpha Actinin 4 with ab231247 at 20 µg/ml in immunohistochemical analysis. DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin 4 antibody (ab231247)Paraffin-embedded human stomach tissue stained for alpha Actinin 4 with ab231247 at 20 µg/ml in immunohistochemical analysis. DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin 4 antibody (ab231247)Paraffin-embedded human kidney tissue stained for alpha Actinin 4 with ab231247 at 20 µg/ml in immunohistochemical analysis. DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin 4 antibody (ab231247)Paraffin-embedded human breast cancer tissue stained for alpha Actinin 4 with ab231247 at 20 µg/ml in immunohistochemical analysis. DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin 4 antibody (ab231247)Paraffin-embedded human bile duct cancer (cholangiocarcinoma) tissue stained for alpha Actinin 4 with ab231247 at 20 µg/ml in immunohistochemical analysis. DAB staining.
-
Western blot - Anti-alpha Actinin 4 antibody (ab231247)Anti-alpha Actinin 4 antibody (ab231247) at 2 µg/ml + Recombinant human alpha Actinin 4 protein
Predicted band size: 105 kDa
