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Anti-Mad2L1 antibody [17D10] (ab10691)

Key features and details

  • Mouse monoclonal [17D10] to Mad2L1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Mad2L1 antibody [17D10]
    See all Mad2L1 primary antibodies

  • Description

    Mouse monoclonal [17D10] to Mad2L1

  • Host species

    Mouse

  • Specificity

    Western blot with a variety of human cell lines confirms that the antibody is specific for Mad2.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant human Mad2.

  • Positive control

    • WB: HAP1, HeLa and A431 cell lysates

Properties

Images

  • Western blot - Anti-Mad2L1 antibody [17D10] (ab10691)
    Western blot - Anti-Mad2L1 antibody [17D10] (ab10691)
    All lanes : Anti-Mad2L1 antibody [17D10] (ab10691) at 2 µg/ml

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : Mad2L1 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : A431 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 24.6 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab10691 observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab10691 was shown to specifically react with in wild-type HAP1 cells as signal was lost in MAD2L1 knockout cells. Wild-type and MAD2L1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab10691 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 2 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L1 antibody [17D10] (ab10691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L1 antibody [17D10] (ab10691)

    ab10691 staining Mad2L1 in colon cancer tissue, after antigen retreival, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

  • Western blot - Anti-Mad2L1 antibody [17D10] (ab10691)
    Western blot - Anti-Mad2L1 antibody [17D10] (ab10691)

    Western blot using ab10691 at 1/500.

    Lane 1: Hela Nuclear
    Lane 2: Hela
    Lane 3: A431
    Lane 4: Jurkat
    Lane 5: 293

    Mad2L1 predicted size: 24.6kD.

    Secondary ab: Rabbit polyclonal to Mouse IgG H&L (HRP) ab6728 (1/5000)
    Exposure time: 1min
    20µg of lysate per lane.

    Western blot using ab10691 at 1/500. Lane 1: Hela Nuclear Lane 2: Hela Lane 3: A431 Lane 4: Jurkat Lane 5: 293 Mad2L1 predicted size: 24.6kD. Secondary ab: anti-mouse HRP (ab6728) (1/5000) Exposure time: 1min 20µg of lysate per lane.

  • Western blot - Anti-Mad2L1 antibody [17D10] (ab10691)
    Western blot - Anti-Mad2L1 antibody [17D10] (ab10691) This image is courtesy of an Abreview submitted by Saira Sarfraz
    Mad2L1 antibody (ab10691) at 1/1000 (in TBS for 90 minutes at 22°C) + whole tissue lysate of Human liver biopsy (chronic hepatitis C - (HepC) or non-viral (NVH)) patients at 50µg.

    Secondary Antibody: An HRP-conjugated Goat anti-mouse polyclonal (1/100000).

    Blocking Step: 5% Milk for 16 hours at 4°C

    See Abreview

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