Anti-Mad2L1 antibody [EPR9853(B)] (ab150371)
![Anti-Mad2L1 antibody [EPR9853(B)] (ab150371)](https://www.abcam.com/ps/products/150/ab150371/Images/ab150371-368171-anti-mad2l-1-antibody-epr9853b-western-blot-knockout-hap1-mad2l1-hela-a431-human.jpg "Anti-Mad2L1 antibody [EPR9853(B)] (ab150371)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9853(B)] to Mad2L1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Mad2L1 antibody [EPR9853(B)]
See all Mad2L1 primary antibodies -
Description
Rabbit monoclonal [EPR9853(B)] to Mad2L1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human Mad2L1 aa 150-250 (C terminal). The exact sequence is proprietary.
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Positive control
- 293T, Jurkat, Raji and A431 cell lysates; WB: Wild-type HAP1 whole cell lysate; HeLa whole cell lysate; A431 whole cell lysate
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9853(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Mad2L1 antibody [EPR9853(B)] (ab150371)All lanes : Anti-Mad2L1 antibody [EPR9853(B)] (ab150371) at 1/10000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : MAD2L1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : A431 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 24 kDa
Observed band size: 24 kDaLanes 1 - 4: Merged signal (red and green). Green - ab150371 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab150371 was shown to specifically react with in wild-type HAP1 cells as signal was lost in MAD2L1 knockout cells. Wild-type and MAD2L1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab150371 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Mad2L1 antibody [EPR9853(B)] (ab150371)](https://www.abcam.com/ps/products/150/ab150371/Images/Mad2L1-Primary-antibodies-ab150371-1.jpg)
Western blot - Anti-Mad2L1 antibody [EPR9853(B)] (ab150371)All lanes : Anti-Mad2L1 antibody [EPR9853(B)] (ab150371) at 1/10000 dilution
Lane 1 : 293T cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : Raji cell lysates
Lane 4 : A431 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 24 kDa
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![Anti-Mad2L1 antibody [EPR9853(B)] (ab150371)](https://www.abcam.com/ps/products/150/ab150371/Images/ab150371-3-benefits-of-recombinant-antibodies.png)
Anti-Mad2L1 antibody [EPR9853(B)] (ab150371)
