Anti-Mad2L1 antibody [OTI4D2] (ab114036)
![Anti-Mad2L1 antibody [OTI4D2] (ab114036)](https://www.abcam.com/ps/products/114/ab114036/Images/Mad2L1-Primary-antibodies-ab114036-3.jpg "Anti-Mad2L1 antibody [OTI4D2] (ab114036)")
Key features and details
- Mouse monoclonal [OTI4D2] to Mad2L1
- Suitable for: WB, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG2a
Overview
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Product name
Anti-Mad2L1 antibody [OTI4D2]
See all Mad2L1 primary antibodies -
Description
Mouse monoclonal [OTI4D2] to Mad2L1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant full length protein corresponding to Human Mad2L1 aa 1 to the C-terminus. Produced in HEK-293T cells (NP_002349).
Database link: Q13257 -
Positive control
- WB: HEK-293T cell lysate transfected with or pCMV6-ENTRY Mad2L1 cDNA. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY Mad2L1.
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General notes
Clone OTI4D2 (formerly 4D2).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI4D2 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-Mad2L1 antibody [OTI4D2] (ab114036)All lanes : Anti-Mad2L1 antibody [OTI4D2] (ab114036) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with the pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with the pCMV6-ENTRY Mad2L1 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 24 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-Mad2L1 antibody [OTI4D2] (ab114036)](https://www.abcam.com/ps/products/114/ab114036/Images/Mad2L1-Primary-antibodies-ab114036-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Mad2L1 antibody [OTI4D2] (ab114036)COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY Mad2L1 stained for Mad2L1 (green) using ab114036 at 1/100 dilution in ICC/IF.
