Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4410] to alpha Actinin 4
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-alpha Actinin 4 antibody [EPR4410]
See all alpha Actinin 4 primary antibodies
Description

Rabbit monoclonal [EPR4410] to alpha Actinin 4
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to residues in Human alpha Actinin 4.
Positive control
- HeLa, MCF-7, C2C12, A673, and L6 cell lysates; Human kidney tissue
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4410
Isotype

IgG
Research areas

Western blot - Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)

All lanes : Anti-alpha Actinin 4 antibody [EPR4410] (ab108201) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate at 40 µg
Lane 2 : ACTN4 (alpha Actinin 4) knockout HAP1 whole cell lysate at 40 µg
Lane 3 : HeLa whole cell lysate at 20 µg

Predicted band size: 105 kDa
Observed band size: 105 kDa

Lanes 1 - 3: Merged signal (red and green). Green - ab108201 observed at 105 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab108201 was shown to recognize alpha Actinin 4 in wild-type HAP1 cells as signal was lost at the expected MW in ACTN4 (alpha Actinin 4) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ACTN4 (alpha Actinin 4) knockout samples were subjected to SDS-PAGE. Ab108201 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)

All lanes : Anti-alpha Actinin 4 antibody [EPR4410] (ab108201) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : C2C12 cell lysate
Lane 4 : A673 cell lysate
Lane 5 : L6 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 105 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)

ab108201, at 1/100 dilution, staining alpha Actinin 4 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-alpha Actinin 4 antibody [EPR4410] (ab108201)

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