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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Crosslinking

Anti-Filamin B antibody (ab97457)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-Filamin B antibody (ab97457)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Filamin B
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Filamin B antibody
    See all Filamin B primary antibodies
  • Description

    Rabbit polyclonal to Filamin B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to Human Filamin B aa 30-239.
    Database link: O75369

  • Positive control

    • WB: 293T, A431, H1299, HeLa and HepG2 whole cell lysate (ab7900) IF: HeLa cells
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Crosslinking
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins

Images

  • Western blot - Anti-Filamin B antibody (ab97457)
    Western blot - Anti-Filamin B antibody (ab97457)
    Anti-Filamin B antibody (ab97457) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 278 kDa



    5% SDS PAGE.

    Secondary antibody - goat anti-rabbit HRP (ab6721)

  • Western blot - Anti-Filamin B antibody (ab97457)
    Western blot - Anti-Filamin B antibody (ab97457)
    Anti-Filamin B antibody (ab97457) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg

    Predicted band size: 278 kDa



    5% SDS PAGE.

     

    Secondary antibody - goat anti-rabbit HRP (ab6721)

  • Immunocytochemistry/ Immunofluorescence - Anti-Filamin B antibody (ab97457)
    Immunocytochemistry/ Immunofluorescence - Anti-Filamin B antibody (ab97457)

    ab97547, at a 1/200 dilution, staining Filamin B in paraformaldehyde-fixed HeLa cells by Immunofluorescence analysis. Right image is merged with a DNA probe.

  • Immunocytochemistry/ Immunofluorescence - Anti-Filamin B antibody (ab97457)
    Immunocytochemistry/ Immunofluorescence - Anti-Filamin B antibody (ab97457) Image from Clark R et al., Mol Cell Proteomics 2013 12: 194-206. . Fig 5.; doi: 10.1074/mcp.M112.019745. Epub 2012 Oct 28.

    Immunofluorescence analysis of Human trabecular meshwork cells, staining Filamin B with ab97457.

    Left panel: Filamin B staining alone. Right panel: Filamin B staining (red) merged with F-actin staining (green).

    Cells were treated with dexamethasone, before fixing in paraformaldehyde and permeabilizing wih 0.2% Triton X-100. Cells were incubated with primary antibody (10 µg/ml) and AlexaFluor®488-conjugated phalloidin. Filamin B staining was detected using an AlexaFluor®546-conjugated goat anti-rabbit IgG.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Filamin B antibody (ab97457)

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    Anti-Filamin B antibody (ab264226)

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    Anti-Filamin B antibody (ab224334)

    Applications: ICC/IF, IHC-P, WB

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    Anti-Filamin B antibody (ab84905)

    Applications: IHC-P

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    Anti-Filamin B antibody (ab264227)

    Applications: IHC-P, WB

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