Human MIP5 Matched Antibody Pair Kit (ab222260)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 15.6 pg/ml - 2000 pg/ml
Overview
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Product name
Human MIP5 Matched Antibody Pair Kit
See all Macrophage inflammatory protein 5 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
12.6 pg/ml -
Range
15.6 pg/ml - 2000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human MIP5 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human MIP5.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab217774. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human MIP5 Capture Antibody 2 x 50µg 1 x 50µg Human MIP5 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human MIP5 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15. -
Tissue specificity
Most abundant in heart, skeletal muscle and adrenal gland. Lower levels in placenta, liver, pancreas and bone marrow. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are found in high levels in synovial fluids from rheumatoid patients. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-C motif chemokine 15
- C-C motif chemokine ligand 15
- CC chemokine 3
see all -
Database links
- Entrez Gene: 6359 Human
- Omim: 601393 Human
- SwissProt: Q16663 Human
- Unigene: 272493 Human
Images
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Human MIP5 Standard Curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human MIP5 Matched Antibody Pair Kit (ab222260)To learn more about the advantages of recombinant antibodies see here.