Anti-alpha Actinin 4 antibody - N-terminal (ab226189)
Key features and details
- Rabbit polyclonal to alpha Actinin 4 - N-terminal
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-alpha Actinin 4 antibody - N-terminal
See all alpha Actinin 4 primary antibodies -
Description
Rabbit polyclonal to alpha Actinin 4 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla
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Immunogen
Synthetic peptide within Human alpha Actinin 4 aa 1-50 (N terminal). The exact sequence is proprietary.
Database link: O43707 -
Positive control
- WB: HeLa, HEK-293T, TCMK-1 and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226189 was affinity purified using an epitope specific to alpha Actinin 4 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-alpha Actinin 4 antibody - N-terminal (ab226189)All lanes : Anti-alpha Actinin 4 antibody - N-terminal (ab226189) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-alpha Actinin 4 antibody - N-terminal (ab226189)alpha Actinin 4 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226189 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226189 at 0.4 µg/ml.
Lane 1: ab226189 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
