Anti-ARHGEF7 antibody (ab236103)
Key features and details
- Rabbit polyclonal to ARHGEF7
- Suitable for: ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ARHGEF7 antibody
See all ARHGEF7 primary antibodies -
Description
Rabbit polyclonal to ARHGEF7 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIP Human
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Immunogen
Recombinant fragment corresponding to Human ARHGEF7 aa 1-250.
Database link: Q14155 -
Positive control
- ICC/IF: HeLa cells. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ARHGEF7 antibody (ab236103)
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for ARHGEF7 (Green) using ab236103 at 1/250 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI (Blue).
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C.
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Immunoprecipitation - Anti-ARHGEF7 antibody (ab236103)ARHGEF7 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab236103 at 1/200 dilution.
Lane 1: Rabbit control IgG (1 µg) in HeLa whole cell lysate.
Lane 2: ab236103 in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate (10 µg input).
For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
