Anti-BI-1 antibody (ab18852)
Key features and details
- Rabbit polyclonal to BI-1
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-BI-1 antibody -
Description
Rabbit polyclonal to BI-1 -
Host species
Rabbit -
Specificity
ab18852 recognises Bax inhibitor. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Rat
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Immunogen
Synthetic peptide corresponding to Human BI-1.
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Positive control
- rat testis tissue lysate
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General notes
Previously labelled as Bax inhibitor 1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.015% EDTA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BI-1 antibody (ab18852)Anti-BI-1 antibody (ab18852) at 1 µg/ml + rat testis tissue lysate
Predicted band size: 26 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-BI-1 antibody (ab18852)ICC/IF image of ab18852 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18852, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.