Anti-NEAS antibody (ab2558)
Key features and details
- Rabbit polyclonal to NEAS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NEAS antibody
See all NEAS primary antibodies -
Description
Rabbit polyclonal to NEAS -
Host species
Rabbit -
Specificity
This antibody recognizes the 240 kDa full length and 150 kDa N-terminal fragment of alpha Fodrin. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human NEAS. Surrounding amino acid 162.
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General notes
This product was previously labelled as Alpha Fodrin, Cleaved alpha-fodrin (Asp1185)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NEAS antibody (ab2558)All lanes : Anti-NEAS antibody (ab2558) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size: 285 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa, 71 kDa. We are unsure as to the identity of these extra bands.
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Western blot - Anti-NEAS antibody (ab2558)Anti-NEAS antibody (ab2558) at 4 µg/ml + HeLa cell extract
Observed band size: 250 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa (possible cleavage fragment)
