Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

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Anti-NEAS antibody [EPR3830-43] (ab133320)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3830-43] to NEAS
Suitable for: ICC/IF, WB, IHC-P
Reacts with: Human

Product name

Anti-NEAS antibody [EPR3830-43]
See all NEAS primary antibodies
Description

Rabbit monoclonal [EPR3830-43] to NEAS
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human NEAS aa 1150-1250.
Positive control
- HeLa and Jurkat cell lysate; Human breast carcinoma and colon tissues; HeLa cells.
General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Alpha Fodrin, Cleaved alpha-fodrin (Asp1185)

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3830-43
Isotype

IgG
Research areas

Anti-NEAS antibody [EPR3830-43] (ab133320)
Western blot - Anti-NEAS antibody [EPR3830-43] (ab133320)

All lanes : Anti-NEAS antibody [EPR3830-43] (ab133320) at 1/10000 dilution

Lane 1 : HeLa cell lysate + camptothecin
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate + staurosporine
Lane 4 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 285 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEAS antibody [EPR3830-43] (ab133320)

Immunohistochemical analysis of Paraffin Embedded Human breast carcinoma tissue labelling NEAS with ab133320 at 1/500 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEAS antibody [EPR3830-43] (ab133320)

Immunohistochemical analysis of Paraffin Embedded Human colon tissue labelling NEAS with ab133320 at 1/500 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-NEAS antibody [EPR3830-43] (ab133320)

Immunofluorescent analysis of HeLa cells labelling NEAS with ab133320 at 1/250 dilution.
Anti-NEAS antibody [EPR3830-43] (ab133320)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com