Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NEAS antibody [EPR3017] (ab75755)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3017] to NEAS
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-NEAS antibody [EPR3017]
    See all NEAS primary antibodies

  • Description

    Rabbit monoclonal [EPR3017] to NEAS

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human NEAS aa 2450-2550. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa lysates, untreated and treated with Camptothecin; Jurkat whole cell lysate (ab7899), untreated and treated with Staurosporine; TF-1, SH-SY5Y and human brain lysate, treated and untreated HeLA and parental HAP1 whole cell lysates

  • General notes

     

     

     This product was previously labelled as Alpha Fodrin, Cleaved alpha-fodrin (Asp1185)

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR3017

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-NEAS antibody [EPR3017] (ab75755)
    Western blot - Anti-NEAS antibody [EPR3017] (ab75755)
    All lanes : Anti-NEAS antibody [EPR3017] (ab75755) at 1/1000 dilution

    Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HeLa, treated with 1 µM Staurosporine for 3 hours, whole cell lysate
    Lane 3 : Untreated parental HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line), whole cell lysate
    Lane 4 : parental HAP1, treated with 1 µM Staurosporine for 3 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 285 kDa



    Merged signal (red and green). Green -ab75755 observed at 285,150,120 kDa using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) as secondary antibody.

     

    Red - loading control, ab7291 observed at 52 kDa using Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) as secondary antibody.

     

    Ab75755 was shown to react with NEAS in HeLa and parental HAP1 cells. lower signal was observed when treated cells (1 µM Staurosporine for 3h) was used. Ab75755 and ab7291 (Mouse anti alpha Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with ab216773 and ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-NEAS antibody [EPR3017] (ab75755)
    Western blot - Anti-NEAS antibody [EPR3017] (ab75755)
    All lanes : Anti-NEAS antibody [EPR3017] (ab75755) at 1/1000 dilution

    Lane 1 : HeLa cell lysate treated with Camptothecin
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat
    treated with Staurosporine
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 285 kDa
    Observed band size: 285 kDa
    Additional bands at: 120 kDa (possible cleavage fragment), 150 kDa (possible cleavage fragment), 85 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-NEAS antibody [EPR3017] (ab75755)
    Western blot - Anti-NEAS antibody [EPR3017] (ab75755)
    All lanes : Anti-NEAS antibody [EPR3017] (ab75755) at 1/2000 dilution

    Lane 1 : TF-1 cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : human
    brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 285 kDa
    Observed band size: 285 kDa
    Additional bands at: 150 kDa (possible cleavage fragment)

  • Anti-NEAS antibody [EPR3017] (ab75755)
    Anti-NEAS antibody [EPR3017] (ab75755)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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