Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Friday March 05, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3017] to NEAS
Suitable for: WB
Reacts with: Mouse, Human

Product name

Anti-NEAS antibody [EPR3017]
See all NEAS primary antibodies
Description

Rabbit monoclonal [EPR3017] to NEAS
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human NEAS aa 2450-2550. The exact sequence is proprietary.
Positive control
- WB: HeLa lysates, untreated and treated with Camptothecin; Jurkat whole cell lysate (ab7899), untreated and treated with Staurosporine; TF-1, SH-SY5Y and human brain lysate, treated and untreated HeLA and parental HAP1 whole cell lysates
General notes
This product was previously labelled as Alpha Fodrin, Cleaved alpha-fodrin (Asp1185)

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3017
Isotype

IgG
Research areas

Western blot - Anti-NEAS antibody [EPR3017] (ab75755)

All lanes : Anti-NEAS antibody [EPR3017] (ab75755) at 1/1000 dilution

Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HeLa, treated with 1 µM Staurosporine for 3 hours, whole cell lysate
Lane 3 : Untreated parental HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line), whole cell lysate
Lane 4 : parental HAP1, treated with 1 µM Staurosporine for 3 hours, whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 285 kDa

Merged signal (red and green). Green -ab75755 observed at 285,150,120 kDa using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) as secondary antibody.

Red - loading control, ab7291 observed at 52 kDa using Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) as secondary antibody.

Ab75755 was shown to react with NEAS in HeLa and parental HAP1 cells. lower signal was observed when treated cells (1 µM Staurosporine for 3h) was used. Ab75755 and ab7291 (Mouse anti alpha Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with ab216773 and ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-NEAS antibody [EPR3017] (ab75755)

All lanes : Anti-NEAS antibody [EPR3017] (ab75755) at 1/1000 dilution

Lane 1 : HeLa cell lysate treated with Camptothecin
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat
treated with Staurosporine
Lane 4 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 285 kDa
Observed band size: 285 kDa
Additional bands at: 120 kDa (possible cleavage fragment), 150 kDa (possible cleavage fragment), 85 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-NEAS antibody [EPR3017] (ab75755)

All lanes : Anti-NEAS antibody [EPR3017] (ab75755) at 1/2000 dilution

Lane 1 : TF-1 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : human
brain lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 285 kDa
Observed band size: 285 kDa
Additional bands at: 150 kDa (possible cleavage fragment)
Anti-NEAS antibody [EPR3017] (ab75755)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com