Anti-NEAS antibody [EPR3017] - BSA and Azide free (ab239863)
![Anti-NEAS antibody [EPR3017] - BSA and Azide free (ab239863)](https://www.abcam.com/ps/products/239/ab239863/Images/ab239863-403243-anti-neas-antibody-epr3017-bsa-and-azide-free-western-blot.gif "Anti-NEAS antibody [EPR3017] - BSA and Azide free (ab239863)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3017] to NEAS - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NEAS antibody [EPR3017] - BSA and Azide free
See all NEAS primary antibodies -
Description
Rabbit monoclonal [EPR3017] to NEAS - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab239863 is the carrier-free version of ab75755 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab239863 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Alpha Fodrin, Cleaved alpha-fodrin (Asp1185)
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3017 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NEAS antibody [EPR3017] - BSA and Azide free (ab239863)All lanes : Anti-NEAS antibody [EPR3017] (ab75755) at 1/1000 dilution
Lane 1 : HeLa cell lysate treated with Camptothecin
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat treated with Staurosporine
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 285 kDa
Observed band size: 285 kDa
Additional bands at: 120 kDa (possible cleavage fragment), 150 kDa (possible cleavage fragment), 85 kDa. We are unsure as to the identity of these extra bands.This data was developed using ab75755, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-NEAS antibody [EPR3017] - BSA and Azide free (ab239863)](https://www.abcam.com/ps/products/239/ab239863/Images/ab239863-399266-anti-neas-antibody-epr3017-bsa-and-azide-free-western-blot.gif)
Western blot - Anti-NEAS antibody [EPR3017] - BSA and Azide free (ab239863)All lanes : Anti-NEAS antibody [EPR3017] (ab75755) at 1/2000 dilution
Lane 1 : TF-1 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : human brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 285 kDa
Observed band size: 285 kDa
Additional bands at: 150 kDa (possible cleavage fragment)This data was developed using ab75755, the same antibody clone in a different buffer formulation.
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![Anti-NEAS antibody [EPR3017] - BSA and Azide free (ab239863)](https://www.abcam.com/ps/products/239/ab239863/Images/ab239863-3-benefits-of-recombinant-antibodies.png)
Anti-NEAS antibody [EPR3017] - BSA and Azide free (ab239863)
