Lanes 2-6 : Anti-NEAS antibody [D8B7] (ab11755) at 0.5 µg/ml

Lane 1 : MW marker
Lane 2 : Human Brain lysate at 20 µg
Lane 3 : Mouse Brain lysate at 20 µg
Lane 4 : Rat Brain lysate at 20 µg
Lane 5 : 3T3 cell lysate at 20 µg
Lane 6 : HeLa cell lysate at 20 µg

Secondary
Lanes 2-6 : HRP labeled goat anti-mouse IgG

Predicted band size: 297 kDa

ICC staining of purified ab11755 on 3T3 cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 1 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm

IHC staining of purified ab11755 on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. HRP kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm

Lanes 2-3 : Anti-NEAS antibody [D8B7] (ab11755) at 0.1 µg/ml

Lane 1 : MW marker
Lane 2 : Drosophila head lysate at 20 µg
Lane 3 : Drosophila S2 (embryonic) cell lysate at 20 µg

Secondary
Lanes 2-3 : HRP-labeled goat anti-mouse IgG

Predicted band size: 297 kDa

ICC staining of purified ab11755 on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 1 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm

IHC staining of purified ab11755 on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. HRP kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm

Anti-NEAS antibody [D8B7] (ab11755) at 1/1000 dilution + 3T3 whole cell lysate at 30 µg

Secondary
HRP conjugated goat anti-mouse at 1/5000 dilution

Performed under reducing conditions.

Predicted band size: 297 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?


Exposure time: 3 minutes

This image was generated using the ascites version of the product.

See Abreview

IF using ab11755.

This image was generated using the ascites version of the product. 

ab11755 at 1/100 staining mouse gut (small bowel) tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was blocked and incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.

This image was generated using the ascites version of the product. 

See Abreview