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Epigenetics and Nuclear Signaling Histones Variants

Anti-Histone H2A.Z (acetyl K4) antibody [RM221] (ab214725)

Anti-Histone H2A.Z (acetyl K4) antibody [RM221] (ab214725)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM221] to Histone H2A.Z (acetyl K4)
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Histone H2A.Z (acetyl K4) antibody [RM221]
    See all Histone H2A.Z primary antibodies
  • Description

    Rabbit monoclonal [RM221] to Histone H2A.Z (acetyl K4)
  • Host species

    Rabbit
  • Specificity

    ab214725 reacts to Histone H2A.Z acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H2A
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Histone H2A.Z (acetyl K4).
    Database link: P0C0S5

  • Positive control

    • HeLa cells extracts, treated with sodium butyrate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 48% PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    RM221
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • Variants
    • Epigenetics and Nuclear Signaling
    • Histones
    • H2A
    • Variants

Images

  • Western blot - Anti-Histone H2A.Z (acetyl K4) antibody [RM221] (ab214725)
    Western blot - Anti-Histone H2A.Z (acetyl K4) antibody [RM221] (ab214725)
    All lanes : Anti-Histone H2A.Z (acetyl K4) antibody [RM221] (ab214725) at 0.5 µg/ml

    Lane 1 : HeLa cell extracts, treated with sodium butyrate
    Lane 2 : HeLa cell extracts, untreated

    Predicted band size: 14 kDa

  • Anti-Histone H2A.Z (acetyl K4) antibody [RM221] (ab214725)
    Anti-Histone H2A.Z (acetyl K4) antibody [RM221] (ab214725)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Anti-Histone H2A.Z (acetyl K7) antibody [RM222] (ab214730)

    Applications: ELISA, ICC/IF, WB

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