Anti-E2F1 (phospho S337) antibody (ab135549)
Key features and details
- Rabbit polyclonal to E2F1 (phospho S337)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-E2F1 (phospho S337) antibody
See all E2F1 primary antibodies -
Description
Rabbit polyclonal to E2F1 (phospho S337) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic phosphopeptide conjugated to KLH, corresponding to amino acid residues surrounding S337 of Human E2F1.
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Positive control
- T47D, HepG2, and A2058 cell lysates; Human breast carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab135549 is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2 step procedure with control and phosphorylated peptides. The phospho specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-E2F1 (phospho S337) antibody (ab135549)All lanes : Anti-E2F1 (phospho S337) antibody (ab135549) at 1/100 dilution
Lane 1 : T47D cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A2058 cell lysate
Lysates/proteins at 12.5 µg per lane.
Predicted band size: 47 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 (phospho S337) antibody (ab135549)Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human breast carcinoma tissue labelling E2F1 (phospho S337) with ab135549 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and AEC staining.