Anti-Histone H3 antibody (ab70550)
Key features and details
- Rabbit polyclonal to Histone H3
- Suitable for: IHC-P, ICC/IF, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Pig, Xenopus laevis, Drosophila melanogaster, Zebrafish, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide within Human Histone H3 aa 100-135 (C terminal). The exact sequence is proprietary.
Database link: P68431 -
Positive control
- Histones isolated from 293T cells. IHC-P: Human normal colon FFPE tissue sections. ICC/IF: HeLa cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Histone H3 antibody (ab70550) at 0.04 µg/ml
Lane 1 : Histones isolated from 293T cells at 50 µg
Lane 2 : Histones isolated from 293T cells at 15 µg
Lane 3 : Histones isolated from 293T cells at 5 µg
Predicted band size: 15 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 1 second
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse teratoma tissue labelling Histone H3 with ab70550 at 1/1000 (0.2µg/ml). Detection: DAB.
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IHC image of ab70550 staining Histone H3 in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70550, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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ab70550 staining Histone H3 in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab70550 at 2.5μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat anti-rabbit AlexaFluor®488 secondary (ab150077) at 2 μg/ml (shown in green). AlexaFluor®350 WGA was used at a 1/200 dilution and incubated for 1h with the cells, to label plasma membranes (shown in blue). Nuclear DNA was labelled in red with 1.25 μM DRAQ5™ (ab108410), which was added to the secondary antibody mixture. A secondary only negative control is displayed, which indicates that the Histone H3 staining observed is due to primary antibody specificity and not to unspecific binding of the secondary antibody to the cells.
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Detection of Human Histone H3 by Western Blot of Immunprecipitate. ab70550 at 1µg/ml staining Histone H3 isolated from 293T cells, immunoprecipitated using ab70550 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 1 second.