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Anti-Histone H3 (asymmetric di methyl R63, symmetric di methyl R63, methyl R63) antibody [EPR17722] (ab196724)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17722] to Histone H3 (asymmetric di methyl R63, symmetric di methyl R63, methyl R63)
  • Suitable for: PepArr, WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Histone H3 (asymmetric di methyl R63, symmetric di methyl R63, methyl R63) antibody [EPR17722]
    See all Histone H3 primary antibodies

  • Description

    Rabbit monoclonal [EPR17722] to Histone H3 (asymmetric di methyl R63, symmetric di methyl R63, methyl R63)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    PepArr
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and NIH3T3 whole cell lysates. IHC-P: Human kidney tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17722

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Histone H3 (asymmetric di methyl R63, symmetric di methyl R63, methyl R63) antibody [EPR17722] (ab196724)
    Western blot - Anti-Histone H3 (asymmetric di methyl R63, symmetric di methyl R63, methyl R63) antibody [EPR17722] (ab196724)
    Anti-Histone H3 (asymmetric di methyl R63, symmetric di methyl R63, methyl R63) antibody [EPR17722] (ab196724) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% BSA /TBST.

  • Peptide Array - Anti-Histone H3 (asymmetric di methyl R63, symmetric di methyl R63, methyl R63) antibody [EPR17722] (ab196724)
    Peptide Array - Anti-Histone H3 (asymmetric di methyl R63, symmetric di methyl R63, methyl R63) antibody [EPR17722] (ab196724)

    ab196724 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.

  • Western blot - Anti-Histone H3 (asymmetric di methyl R63, symmetric di methyl R63, methyl R63) antibody [EPR17722] (ab196724)
    Western blot - Anti-Histone H3 (asymmetric di methyl R63, symmetric di methyl R63, methyl R63) antibody [EPR17722] (ab196724)
    Anti-Histone H3 (asymmetric di methyl R63, symmetric di methyl R63, methyl R63) antibody [EPR17722] (ab196724) at 1/1000 dilution + NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% BSA /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (asymmetric di methyl R63, symmetric di methyl R63, methyl R63) antibody [EPR17722] (ab196724)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (asymmetric di methyl R63, symmetric di methyl R63, methyl R63) antibody [EPR17722] (ab196724)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Histone H3 (methyl R63, asymmetric di methyl R63, symmetric di methyl R63) with ab196724 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Anti-Histone H3 (asymmetric di methyl R63, symmetric di methyl R63, methyl R63) antibody [EPR17722] (ab196724)
    Anti-Histone H3 (asymmetric di methyl R63, symmetric di methyl R63, methyl R63) antibody [EPR17722] (ab196724)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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