Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Expression system: Escherichia coli
Purity: > 95% SDS-PAGE
Endotoxin level:
Suitable for: SDS-PAGE, HPLC

Product name

Recombinant Mouse PEDF protein
See all PEDF proteins and peptides
Purity

> 95 % SDS-PAGE.
Endotoxin level
Expression system

Escherichia coli
Accession

P97298
Protein length

Protein fragment
Animal free

No
Nature

Recombinant
- Species
  
  Mouse
- Sequence
  
  MDPFFKVPVNKLAAAVSNFGYDLYRLRSSASPTGNVLLSPLSVATALSAL SLGAEHRTESVIHRA LYYDLITNPDIHSTYKELLASVTAPEKNLKSASRIVFERKLRVKSSFVAP LEKSYGTRPRILTGN PRVDLQEINNWVQAQMKGKIARSTREMPSALSILLLGVAYFKGQWVTKFD SRKTTLQDFHLDEDR TVRVPMMSDPKAILRYGLDSDLNCKIAQLPLTGSMSIIFFLPLTVTQNLT MIEESLTSEFIHDID RELKTIQAVLTVPKLKLSFEGELTKSLQDMKLQSLFESPDFSKITGKPVK LTQVEHRAAFEWNEE GAGSSPSPGLQPVRLTFPLDYHLNQPFLFVLRDTDTGALLFIGRILDPSS T
- Predicted molecular weight
  
  46 kDa
- Amino acids
  
  43 to 417

Our Abpromise guarantee covers the use of ab185860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Applications

SDS-PAGE

HPLC
Form

Liquid
Concentration information loading...

Stability and Storage

Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

pH: 7.40
Constituent: 100% PBS

Alternative names
- Cell proliferation-inducing gene 35 protein
- EPC 1
- EPC-1
- EPC1
- OI12
- OI6
- PEDF
- PEDF_HUMAN
- PIG 35
- PIG35
- Pigment epithelium derived factor
- Pigment epithelium-derived factor
- Proliferation inducing protein 35
- Serine (or cysteine) proteinase inhibitor
- serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
- Serpin F1
- Serpin family F member 1
- Serpin peptidase inhibitor
- Serpin peptidase inhibitor clade F member 1
- serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
- SERPINF 1
- Serpinf1
see all
Function

Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Tissue specificity

Retinal pigment epithelial cells and blood plasma.
Sequence similarities

Belongs to the serpin family.
Developmental stage

Expressed in quiescent cells.
Domain

The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.
Post-translational
modifications

The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Cellular localization

Secreted. Melanosome. Enriched in stage I melanosomes.
Target information above from: UniProt accession P36955 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols